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nontargeting sirna (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology nontargeting sirna
Nontargeting Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nontargeting sirna/product/Santa Cruz Biotechnology
Average 90 stars, based on 4 article reviews

nontargeting sirna - by Bioz Stars, 2026-03

90/100 stars

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Association of huntingtin with PSD-95 and SAP102. A, PSD-95 associates with htt in YAC transgenic mouse striatal tissues. Lysates from 4- to 8-week-old mouse striatal tissue were immunoprecipitated with MAB2166 or HD650 (an anti-human htt-specific antibody). The blot was cut into two parts along the 160 kDa marker and probed with anti-htt and anti-PSD-95 antibodies, respectively. The mean band density ratio of PSD-95 to huntingtin for pooled data is shown below (in each experiment, YAC72 and/or YAC128 were run on same gel as YAC18, and band density ratios were normalized to those in YAC18 lane). Significant by one-way ANOVA and Bonferroni's post hoc tests, *p < 0.05 (n = 3). B, SAP102 associates with huntingtin in YAC transgenic mouse striatal tissue. Lysates from 4-week-old YAC transgenic mouse striatal tissue were immunoprecipitated with MAB2166 or HD650. The blot was cut into two parts along the 160 kDa marker and probed with anti-htt and anti-SAP102 antibodies, respectively. Pooled data for SAP102/htt density ratios are shown below representative blot. Ratios are similar across all three genotypes (in each experiment, YAC72 and/or YAC128 were run on same gel as YAC18, and band density ratios were normalized to those in YAC18 lane). p > 0.05 by one-way ANOVA; n = 7 for YAC18 and YAC72, n = 5 for YAC128. C, SAP102 associates with huntingtin in HEK293T cells. Lysates from HEK239T cells transfected with full-length htt15 or htt138, and SAP102 were immunoprecipitated and probed as described in B. Note that SAP102/htt density ratios are similar for htt15 and htt138.

Journal: The Journal of Neuroscience

Article Title: Interaction of Postsynaptic Density Protein-95 with NMDA Receptors Influences Excitotoxicity in the Yeast Artificial Chromosome Mouse Model of Huntington's Disease

doi: 10.1523/JNEUROSCI.2491-09.2009

Figure Lengend Snippet: Association of huntingtin with PSD-95 and SAP102. A, PSD-95 associates with htt in YAC transgenic mouse striatal tissues. Lysates from 4- to 8-week-old mouse striatal tissue were immunoprecipitated with MAB2166 or HD650 (an anti-human htt-specific antibody). The blot was cut into two parts along the 160 kDa marker and probed with anti-htt and anti-PSD-95 antibodies, respectively. The mean band density ratio of PSD-95 to huntingtin for pooled data is shown below (in each experiment, YAC72 and/or YAC128 were run on same gel as YAC18, and band density ratios were normalized to those in YAC18 lane). Significant by one-way ANOVA and Bonferroni's post hoc tests, *p < 0.05 (n = 3). B, SAP102 associates with huntingtin in YAC transgenic mouse striatal tissue. Lysates from 4-week-old YAC transgenic mouse striatal tissue were immunoprecipitated with MAB2166 or HD650. The blot was cut into two parts along the 160 kDa marker and probed with anti-htt and anti-SAP102 antibodies, respectively. Pooled data for SAP102/htt density ratios are shown below representative blot. Ratios are similar across all three genotypes (in each experiment, YAC72 and/or YAC128 were run on same gel as YAC18, and band density ratios were normalized to those in YAC18 lane). p > 0.05 by one-way ANOVA; n = 7 for YAC18 and YAC72, n = 5 for YAC128. C, SAP102 associates with huntingtin in HEK293T cells. Lysates from HEK239T cells transfected with full-length htt15 or htt138, and SAP102 were immunoprecipitated and probed as described in B. Note that SAP102/htt density ratios are similar for htt15 and htt138.

Article Snippet: The control [“scrambled” small interfering RNA (siRNA); sc-36869; or nontargeting siRNA, D-001950-01] and PSD-95 siRNA (sc-42012) and NE–Dlg (neuronal and endocrine DIg) (SAP102) siRNA (sc-42007) were purchased from Santa Cruz Biotechnology or Dharmacon (Thermo Fisher Scientific).

Techniques: Transgenic Assay, Immunoprecipitation, Marker, Transfection

Effect of mutant htt expression on interaction of NR2B with PSD-95 and SAP102. A, Mutant htt expression alters the association of PSD-95 with NR2B in vivo. NR2B was immunoprecipitated from lysates of 8-week-old WT and YAC transgenic mouse striatal tissue using an anti-NR2B antibody (Affinity BioReagents), and proteins were subjected to Western blot analysis. The blot was cut into two parts along the 160 kDa marker and probed with anti-NR2B and anti-PSD-95 antibodies, respectively. The mean band density ratio of PSD-95 to NR2B for pooled data is shown below (in each experiment, YAC72 and/or YAC128 were run on same gel as WT, and band density ratios were normalized to those in WT lane). Significant by one-way ANOVA and Bonferroni's post hoc tests, *p < 0.05, ***p < 0.001 (n = 8 for WT; n = 7 for YAC72 and YAC128). B, Representative blot showing that coimmunoprecipitation of SAP102 with NR2B in 4-week-old YAC mouse striatal tissue is not altered by htt repeat length. Pooled data for the mean band density ratio of SAP102 to NR2B is shown (in each experiment, YAC72 and/or YAC128 were run on same gel as YAC18, and band density ratios were normalized to those in YAC18 lane). There is no significant difference by one-way ANOVA, p > 0.05 (n = 6 for YAC18 and YAC128; n = 5 for YAC72). C, Representative blot showing that coimmunoprecipitation of PSD-95 with NR2A in 8-week-old YAC mouse striatal tissue is not altered by htt polyQ repeat length. Pooled data for the mean band density ratio of PSD-95 to NR2A is shown. There is no significant difference by one-way ANOVA, p > 0.05 (n = 3 for YAC18; n = 5 for WT and YAC72; n = 4 for YAC128).

Journal: The Journal of Neuroscience

Article Title: Interaction of Postsynaptic Density Protein-95 with NMDA Receptors Influences Excitotoxicity in the Yeast Artificial Chromosome Mouse Model of Huntington's Disease

doi: 10.1523/JNEUROSCI.2491-09.2009

Figure Lengend Snippet: Effect of mutant htt expression on interaction of NR2B with PSD-95 and SAP102. A, Mutant htt expression alters the association of PSD-95 with NR2B in vivo. NR2B was immunoprecipitated from lysates of 8-week-old WT and YAC transgenic mouse striatal tissue using an anti-NR2B antibody (Affinity BioReagents), and proteins were subjected to Western blot analysis. The blot was cut into two parts along the 160 kDa marker and probed with anti-NR2B and anti-PSD-95 antibodies, respectively. The mean band density ratio of PSD-95 to NR2B for pooled data is shown below (in each experiment, YAC72 and/or YAC128 were run on same gel as WT, and band density ratios were normalized to those in WT lane). Significant by one-way ANOVA and Bonferroni's post hoc tests, *p < 0.05, ***p < 0.001 (n = 8 for WT; n = 7 for YAC72 and YAC128). B, Representative blot showing that coimmunoprecipitation of SAP102 with NR2B in 4-week-old YAC mouse striatal tissue is not altered by htt repeat length. Pooled data for the mean band density ratio of SAP102 to NR2B is shown (in each experiment, YAC72 and/or YAC128 were run on same gel as YAC18, and band density ratios were normalized to those in YAC18 lane). There is no significant difference by one-way ANOVA, p > 0.05 (n = 6 for YAC18 and YAC128; n = 5 for YAC72). C, Representative blot showing that coimmunoprecipitation of PSD-95 with NR2A in 8-week-old YAC mouse striatal tissue is not altered by htt polyQ repeat length. Pooled data for the mean band density ratio of PSD-95 to NR2A is shown. There is no significant difference by one-way ANOVA, p > 0.05 (n = 3 for YAC18; n = 5 for WT and YAC72; n = 4 for YAC128).

Techniques: Mutagenesis, Expressing, In Vivo, Immunoprecipitation, Transgenic Assay, Western Blot, Marker

Tat–NR2B9c (9c) disrupts coimmunoprecipitation of NR2B with PSD-95 and SAP102 in cultured MSNs. A, Representative blot showing that 1 μm Tat–NR2B9c disrupts co-IP of NR2B with PSD-95 in YAC128 cultured MSNs. B, Representative blot showing that 1 μm Tat–NR2B9c disrupts co-IP of NR2B with SAP102 in YAC128 cultured MSNs. C, Representative blot showing that 200 nm Tat–NR2B9c disrupts co-IP of NR2B with both PSD-95 and SAP102 in YAC128 cultured MSNs. A–C, The concentration of Tat–NR2BAA (AA) used in each experiment matched that of the Tat–NR2B9c (9c). D, The pooled data of the reduction of either PSD-95 or SAP102 (MAGUK) to NR2B band intensity ratio by both 200 nm and 1 μm Tat–NR2B9c concentrations (normalized to its paired Tat–NR2BAA at the same concentration as Tat–NR2B9c). For the four bars from left to right, *p < 0.05, n = 3; *p < 0.05, n = 8; *p < 0.05, n = 4; **p < 0.01, n = 3, compared with its paired Tat–NR2BAA at the same concentration (data not shown, all equal to 1), by paired one-sample t test, respectively.

Journal: The Journal of Neuroscience

Article Title: Interaction of Postsynaptic Density Protein-95 with NMDA Receptors Influences Excitotoxicity in the Yeast Artificial Chromosome Mouse Model of Huntington's Disease

doi: 10.1523/JNEUROSCI.2491-09.2009

Figure Lengend Snippet: Tat–NR2B9c (9c) disrupts coimmunoprecipitation of NR2B with PSD-95 and SAP102 in cultured MSNs. A, Representative blot showing that 1 μm Tat–NR2B9c disrupts co-IP of NR2B with PSD-95 in YAC128 cultured MSNs. B, Representative blot showing that 1 μm Tat–NR2B9c disrupts co-IP of NR2B with SAP102 in YAC128 cultured MSNs. C, Representative blot showing that 200 nm Tat–NR2B9c disrupts co-IP of NR2B with both PSD-95 and SAP102 in YAC128 cultured MSNs. A–C, The concentration of Tat–NR2BAA (AA) used in each experiment matched that of the Tat–NR2B9c (9c). D, The pooled data of the reduction of either PSD-95 or SAP102 (MAGUK) to NR2B band intensity ratio by both 200 nm and 1 μm Tat–NR2B9c concentrations (normalized to its paired Tat–NR2BAA at the same concentration as Tat–NR2B9c). For the four bars from left to right, *p < 0.05, n = 3; *p < 0.05, n = 8; *p < 0.05, n = 4; **p < 0.01, n = 3, compared with its paired Tat–NR2BAA at the same concentration (data not shown, all equal to 1), by paired one-sample t test, respectively.

Techniques: Cell Culture, Co-Immunoprecipitation Assay, Concentration Assay

PSD-95 but not SAP102 knockdown by siRNA reduces NMDA toxicity in YAC128 MSNs but not WT MSNs. A, Representative Western blots and pooled data showing the knockdown of PSD-95 expression in WT and YAC128 MSNs 72 h after addition of PSD-95 siRNA but not control siRNA to culture medium. **p < 0.01, ***p < 0.001, by one-way ANOVA and Bonferroni's post hoc tests (n = 3 for WT and YAC128). B, Representative photomicrographs showing TUNEL- and Hoechst-stained WT and YAC128 MSNs pretreated with control siRNA (top) or PSD-95 siRNA (bottom) 72 h before 10 min challenge of NMDA. C, The effect of PSD-95 knockdown by siRNA on NMDA-induced toxicity in WT and YAC128 MSNs. Average proportion of apoptotic MSNs are shown (mean values calculated after subtraction of percentage apoptosis in NMDA-untreated condition for each experiment). Two-way ANOVA revealed significant difference for genotype, treatment, and interaction, n = 3; F(1,20) = 22, p < 0.001 for genotype; F(4,20) = 25, p < 0.001 for treatment; F(4,20) = 29, p < 0.001 for interaction (***p < 0.001 by Bonferroni's post hoc tests). D, Representative Western blots showing the knockdown of SAP102 expression in WT and YAC128 MSNs 72 h after addition of SAP102 siRNA but not control siRNA to culture medium (n = 3 for WT and YAC128). E, The effect of SAP102 knockdown on NMDA-induced toxicity in WT and YAC128 MSNs. Average proportion of apoptotic MSNs are shown (mean values calculated after subtraction of percentage apoptosis in NMDA-untreated condition for each experiment). Two-way ANOVA revealed significant difference for genotype, treatment, and interaction; n = 3 for WT and n = 4 for YAC128; F(1,25) = 52, p < 0.001 for genotype; F(4,25) = 11, p < 0.001 for treatment; F(4,25) = 14, p < 0.001 for interaction (***p < 0.001 by Bonferroni's post hoc tests). 9c, NR2B9c, N, NMDA.

Journal: The Journal of Neuroscience

Article Title: Interaction of Postsynaptic Density Protein-95 with NMDA Receptors Influences Excitotoxicity in the Yeast Artificial Chromosome Mouse Model of Huntington's Disease

doi: 10.1523/JNEUROSCI.2491-09.2009

Figure Lengend Snippet: PSD-95 but not SAP102 knockdown by siRNA reduces NMDA toxicity in YAC128 MSNs but not WT MSNs. A, Representative Western blots and pooled data showing the knockdown of PSD-95 expression in WT and YAC128 MSNs 72 h after addition of PSD-95 siRNA but not control siRNA to culture medium. **p < 0.01, ***p < 0.001, by one-way ANOVA and Bonferroni's post hoc tests (n = 3 for WT and YAC128). B, Representative photomicrographs showing TUNEL- and Hoechst-stained WT and YAC128 MSNs pretreated with control siRNA (top) or PSD-95 siRNA (bottom) 72 h before 10 min challenge of NMDA. C, The effect of PSD-95 knockdown by siRNA on NMDA-induced toxicity in WT and YAC128 MSNs. Average proportion of apoptotic MSNs are shown (mean values calculated after subtraction of percentage apoptosis in NMDA-untreated condition for each experiment). Two-way ANOVA revealed significant difference for genotype, treatment, and interaction, n = 3; F(1,20) = 22, p < 0.001 for genotype; F(4,20) = 25, p < 0.001 for treatment; F(4,20) = 29, p < 0.001 for interaction (***p < 0.001 by Bonferroni's post hoc tests). D, Representative Western blots showing the knockdown of SAP102 expression in WT and YAC128 MSNs 72 h after addition of SAP102 siRNA but not control siRNA to culture medium (n = 3 for WT and YAC128). E, The effect of SAP102 knockdown on NMDA-induced toxicity in WT and YAC128 MSNs. Average proportion of apoptotic MSNs are shown (mean values calculated after subtraction of percentage apoptosis in NMDA-untreated condition for each experiment). Two-way ANOVA revealed significant difference for genotype, treatment, and interaction; n = 3 for WT and n = 4 for YAC128; F(1,25) = 52, p < 0.001 for genotype; F(4,25) = 11, p < 0.001 for treatment; F(4,25) = 14, p < 0.001 for interaction (***p < 0.001 by Bonferroni's post hoc tests). 9c, NR2B9c, N, NMDA.

Techniques: Western Blot, Expressing, TUNEL Assay, Staining

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